4 edition of Captan-induced reversions in bacteria found in the catalog.
Captan-induced reversions in bacteria
Gayle M. Nii LoPiccolo
Written in English
|Statement||by Gayle M. Nii LoPiccolo.|
|LC Classifications||Microfilm 40623 (Q)|
|The Physical Object|
|Pagination||v, 58 leaves|
|Number of Pages||58|
|LC Control Number||89894050|
Involves the transfer of naked DNA from the environment to the recipent cell, cells rupture during the stationary and death phase, the chromosome breaks into small pieces and explodes through the ruptured cell wall, recipeitn "competent" cell picks up piece of the naked DNA, the naked DNA is integrated onto the recipeint chromosome, (replaces the homologous gene on the cromose to the recipeint. Conversions and reversions are reported to be common in studies from low TB prevalence countries –. Conversions indicate recent infections with increased risk of progression whereas reversions may indicate bacterial clearance. There is limited information regarding conversions and reversions of QFT-GIT test results among pulmonary TB patients.
The Chemical Activities of Bacteria. By E. F. GALE, B.A., Ph.D. 5 x 7* in., pp. with II text-figures. London: University Tutorial Press, Ltd. Price 8^. bd. Because of their intense metabolism and the ease with which they can be handled under a wide range of controlled external conditions, bacteria are ideal organisms with which to. Experimental approaches to evolution provide indisputable evidence of evolution by directly observing the process at work. Experimental evolution deliberately duplicates evolutionary processes―forcing life histories to evolve, producing adaptations to stressful environmental conditions, and generating lineage splitting to create incipient s: 1.
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CAPTAN-INDUCED REVERSIONS IN BACTERIA by Gayle M. Nii LoPiccolo A Thesis Submitted to the Faculty of The College of Graduate Studies in partial fulfillment of the Degree of Master of Arts Western Michigan University Kalamazoo, Michigan April Reproduced with permission of the copyright owner.
Further reproduction prohibited without by: 4. By Gayle M. Nii LoPiccolo, Published on 04/01/ Recommended Citation. LoPiccolo, Gayle M. Nii, "Captan-Induced Reversions in Bacteria" ().Cited by: 4. To explain our negative results with captan in the HMA, either lack of suffi- cient uptake, inactivation of mutagenicity (see below) or lack of exposure of 12 bacteria in the peritoneal cavity may have been involved.
None of these limita- tions applied to SZN since it induced many reversions at doses twenty to a fold below those of by: Captan (N-(trichloromethylthio)-3a,4,7,7a-tetrahydrophthalimide) and folpet (N-(trichloromethylthio)-phthalimide), two closely related fungicides, were tester for mutagenicity in Drosophila compounds were administered either by injection into adult males or by feeding to larvae.
The following types of genetic damage were measured: (1) complete and mosaic Cited by: Introduction. In bacterial mutagenicity screening tests for chemicals Escherichia coli tester strain WP2uvrA or WP2uvrA/pKM is recommended as a supplement to the standard Salmonella typhimurium tester strains.
Some mutagens induce base substitutions in tester strains but not in urium tester strains TA, TA and TA (Matsushima et al., ; Gatehouse et al., Cited by: The Ames test uses bacteria as a very sensitive biological indicator of whether or not a substance can cause a change in DNA sequence.
Dr Bruce Ames started out with a bacterial strain with a mutation in a gene required to make the amino acid histidine. Because of the defect in this gene, the bacteria can only grow on food that provides histidine. Plasmids, and sometimes bacterial chromosomes, are transferred between two cells during bacterial mating.
Recently it has become possible to artificially synthesize whole bacterial chromosomes and re-introduce them into the corresponding bacterial cells. This opens the way for improved genetic engineering of bacteria.
Reversions of trpE65 to Trp + phenotype and tyrA14 to Tyr + in E. coli B/r WP2 (Ohta et al., ) and WU derivatives (Bockrath et al., ), respectively, are analogous to K12 AB mutation detection systems.
Both trpE65 and tyrA14 are ochre mutations in genes coding for enzymes involved in tryptophane and tyrosine biosynthesis. L.J. Reha-Krantz, in Brenner's Encyclopedia of Genetics (Second Edition), Reversion Frequencies.
The reversion frequency for the culture illustrated in Figure 1 is 5 Trp + revertants per 10 8 cells. Reversion frequencies, however, cannot be determined from a single culture since a mutation that restores function can occur at any time during growth of the culture. Round bacteria are called cocci (singular coccus).
Rod shaped bacteria are called bacilli (singular bacillus). Other shapes will be considered later in the course. Bacteria are very difficult to study microscopically unless stained.
The staining characteristics of bacteria. Influence of bacterial growth of the overnight culture on the captan-and folpet-induced reversion in the Ames test Carmen Barrueco and Eduardo de la Peña Instituto de Edafología y Biología Vegetal.
Serrano dpdo, Madrid. Spain The influence of the stage of bacterial growth of the over. plate surface to achieve isolation of bacteria. Incubate at °C for 24 hours to allow bacterial growth; all tests should be performed on hour cultures.
Using a sterile needle or small loop, pick individual distinct bacterial colonies. Use of a dissecting scope can aid. Bacteria are generally studied when fixed and stained.
Smears or films of bacterial cultures and clinical specimens are usually fixed by heat, the slide being first throughly dried in air and then heated gently in a flame.
Vegetative bacteria are thereby killed, attached to the surface of the slide and preserve from undergoing autolytic changes. The evolutionary significance of stress-induced mutagenesis was evaluated by studying mutagenesis in aging colonies (MAC) of Escherichia coli natural isolates.
A large fraction of isolates exhibited a strong MAC, and the high MAC variability reflected the diversity of selective pressures in ecological niches. MAC depends on starvation, oxygen, and RpoS and adenosine 3′,5′. the bacterial cell wall, as well as, to some extent, the chemical composition.
Gram-Positive bacteria have thick, dense, relatively non-porous walls, while Gram-Negative bacteria have thin walls surrounded by lipid-rich membranes. Some non-bacterial organisms with thick cell walls (e.g., some yeasts) also stain Gram-Positive.
Bacterial Resistance to Antibiotics – From Molecules to Man is a valuable source of up-to-date information for medical practitioners, researchers, academics, and professionals in public health, pharmaceuticals, microbiology, and related fields.
Hfr strains of bacteria: A. do not have an "F" (fertility) factor. have an "F" factor plasmid. have an "F" factor integrated in the bacterial chromosome. transfer the genetic information to other bacteria with high frequency Match the following terms () with their respective meanings (A-F).
Confirmation of reversions in the hisC gene at amyE. To determine if reversions were occurring in the copy of hisC at amyE, twenty-eight colonies, twenty-four head-on and four co-directional, from plates with IPTG were sequenced.
The revertant colonies were grown up in selective (histidine- spectinomycin+) media and genomic DNA was prepared. Full text Full text is available as a scanned copy of the original print version.
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Links to PubMed are also available for Selected References. Mutagenesis of the large subunit (LS) of the potato ADP-glucose pyrophosphorylase generated an enzyme, P52L, that was insensitive to 3-phosphoglycerate (3-PGA).
To identify additional residues involved in 3-PGA interaction, we subjected P52L LS DNA to a second round of mutagenesis and identified second-site revertants by their ability to restore glycogen accumulation as assessed by.
Biochemistry All Publications/Website. OR SEARCH CITATIONS.The mutant Salmonella bacteria that are used in the Ames test can't make their own histidine. Histidine (His) is an essential amino acid that the bacteria need to live.Bridges BA, Ashwood-Smith MJ, Munson RJ. On the nature of the lethal and mutagenic action of ultraviolet light on frozen bacteria.
Proc R Soc Lond B Biol Sci. Aug 15; ()– Osborn M, Person S, Phillips S, Funk F. A determination of mutagen specificity in bacteria using nonsense mutants of bacteriophage T4.